HEAT-INDUCED OXIDATIVE STRESS AND INFLAMMATION IN RATS IN RELATION TO AGE

Jovana Ilievska, Viktor Cicimov, Emilija Antova, Icko Gjorgoski, Nikola Hadzy-Petrushev, Mitko Mladenov

Heat stress is implied to be an environmental factor for stimulating reactive oxygen species (ROS) production because of the resemblance in responses observed following heat stress with those occurring in the state of oxidative stress. To investigate the protein oxidation, lipid peroxidation and inflammation related to aging and heat stress we determined the levels of sulfhydryl (SH) groups, carbonyl (CO) groups, malondialdehyde (MDA) and tumor-necrosis factor-alpha (TNF-α) in the plasma of rats at two different ages (14 days and 18 months). The results showed that heat stress increased the plasma levels of CO, MDA and TNF-α and lowered the levels of SH. The concentration of TNF-α and MDA remained unchanged in relation to aging. Moreover the process of aging was characterized by increased protein oxidation, according to the observed decrease in the level of sulfhydryl groups and the increase in carbonyl groups plasma concentration. Our study showed that acute heat stress leads to increased lipid peroxidation, protein oxidation and inflammation in rats. Additionally, it was evident that young rats possess greater ability to cope with heat-induced oxidative stress and inflammation compared to the ability of aged animals, in which a pre-existing state of increased protein oxidation probably augments the cellular damage related to the heat exposure.

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